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C5608

Sigma-Aldrich

Collagen from rabbit skin

Bornstein and Traub Type I, powder

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

biological source

rabbit skin

Quality Level

description

acid soluble

form

powder

technique(s)

cell culture | stem cell: suitable

solubility

aqueous acid: ≤10 mg/mL
aqueous sodium acetate buffer: soluble (0.01-0.5M)

UniProt accession no.

storage temp.

2-8°C

Gene Information

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C9879C9301C9791
vibrant-m

C5608

Collagen from rabbit skin

vibrant-m

C9791

Collagen from calf skin

technique(s)

cell culture | stem cell: suitable

technique(s)

ELISA: suitable, activity assay: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

solid

solubility

aqueous acid: ≤10 mg/mL, aqueous sodium acetate buffer: soluble (0.01-0.5M)

solubility

-

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

solubility

0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

UniProt accession no.

Q28668

UniProt accession no.

P02459

UniProt accession no.

P02460

UniProt accession no.

P02453

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen-type 1 may be used in research of Idiopathic pulmonary fibrosis (IPF). Robust expression of collagen-type 1 is one distinctive feature of IPF. Additionally, collagen-type 1 has been used in studies on the effect of endoplasmic reticulum (ER) stress from IPF on myofibroblastic differentiation of lung fibroblasts. Collage-type 1 soluble in acidic solution produces three dimensional scaffolding useful in bioengineering and cell culture applications where biomaterials are needed to replace native collagen extracellular matrices.

Collagen Type I has been used as a scaffold for the growth in vitro of stem cells in a wide variety of biomaterial engineering studies.

Preparation Note

Prepared with modification of Gallop, P.M.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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