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C5838

Sigma-Aldrich

Monoclonal Anti-Actin–Cy3 antibody produced in mouse

clone AC-40, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-Actin
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-40, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat

technique(s)

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
C6198A5691A3853
conjugate

CY3 conjugate

conjugate

CY3 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

AC-40, monoclonal

clone

1A4, monoclonal

clone

1A4, monoclonal

clone

AC-40, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

mol wt

antigen 42 kDa

mol wt

antigen ~42 kDa

mol wt

antigen ~42 kDa

mol wt

-

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General description

Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.

Immunogen

synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone

Application

Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biochem/physiol Actions

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Legal Information

Distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Erin Faught et al.
Scientific reports, 8(1), 18081-18081 (2018-12-29)
The mineralocorticoid receptor (MR) in mammals mediates the effects of aldosterone in regulating fluid balance and potassium homeostasis. While MR signalling is essential for survival in mammals, there is no evidence that MR has any physiological role in ray-finned fish.
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Gunning P, et al.
Molecular and Cellular Biology, 3(5), 787-795 (1983)
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
Wentao Shi et al.
Molecular medicine reports, 20(3), 2763-2773 (2019-07-20)
Ectomesenchymal stem cells (EMSCs) represent a type of adult stem cells derived from the cranial neural crest. These cells are capable of self‑renewal and have the potential for multidirectional differentiation. Tissue transglutaminase type 2 (TG2) is a ubiquitously expressed member
Xiao-Yan Wen et al.
JCI insight, 3(24) (2018-12-21)
Sialic acids are important components of glycoproteins and glycolipids essential for cellular communication, infection, and metastasis. The importance of sialic acid biosynthesis in human physiology is well illustrated by the severe metabolic disorders in this pathway. However, the biological role

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