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C8534

Sigma-Aldrich

Anti-Chorionic Gonadotropin (α+β subunits) (HCG) antibody produced in rabbit

whole antiserum

Synonym(s):

Chorionic Gonadotropin Antibody, HCG Antibody - Anti-Chorionic Gonadotropin (α+β subunits) (HCG) antibody produced in rabbit, Hcg Antibodies, Hcg Antibody

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunohistochemistry: 1:100 using human placental tissue
radioimmunoassay: 1:50,000

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Human Chorionic Gonadotropin (HCG) is a hormone detected in the blood and urine during pregnancy. The peak levels of HCG are detected around Week 12 of gestation and diminish during remaining period of pregnancy. HCG is composed of α and β subunits. There are five variants of HCG that have been identified; HCG, hyperglycosylated HCG, sulphated HCG, free β subunit and pituitary HCG. The important functions of HCG include implantation, angiogenesis, progesterone production, regulation of immune cells and cytotrophoblast differentiation and development of placenta
Anti-Chorionic Gonadotropin (α + α subunits) antibody is specific for both α and β subunits of human chorionic gonadotropin.

Immunogen

HCG

Application

Anti-Chorionic Gonadotropin (α + α subunits) antibody may be used for RIA at a minimum working dilution of 1:50,000. For immunohistochemistry, the recommended working dilution is 1:100-1:200. The antibody was also used for immunofluorescence at a working dilution of 1:50 and for ELISA.

Reconstitution

Dilute with buffer: 0.01 M phosphate, pH 7.8, 0.15 M sodium chloride, 0.5% bovine serum albumin and 0.1 sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

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Rui Zhao et al.
Oncology letters, 16(4), 4271-4278 (2018-09-11)
Hormones and their receptors affect the development process of gastric cancer. Previous studies have revealed that human chorionic gonadotropin (hCG) is expressed in gastric cancer tissue. However, the mechanism by which hCG exerts its effects on gastric cancer cells had
Biological functions of hCG and hCG-related molecules
Cole LA
Reproductive Biology and Endocrinology, 8, doi: 10-doi: 10 (2010)
hCG, the wonder of today's science
Cole LA
Reproductive Biology and Endocrinology, 10, doi: 10-doi: 10 (2010)
Anupam Basu et al.
Analytical biochemistry, 366(2), 175-181 (2007-06-02)
The development of a simultaneous multianalyte immunoassay for the detection of progesterone and human chorionic gonadotropin (hCG) in serum is described. In this simultaneous multianalyte assay, two different enzymes, viz. horse radish peroxidase (HRP) and alkaline phosphatase (ALP), were used
R K Smith et al.
Microscopy research and technique, 64(1), 54-62 (2004-08-03)
Immunocytochemical confocal laser scanning microscope images of the monolayer of cells lining the intervillus space at the basal plate of term placentae were analysed using stereology. Immunoreactively-distinct regions of this mosaic layer were measured. In basal plate from healthy pregnancies

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