C8740
CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, 10× concentrate
Synonym(s):
bacterial cell lysis reagent
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About This Item
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Quality Level
form
liquid
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Application
TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
Features and Benefits
- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Certificates of Analysis (COA)
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