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CelLytic Express

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Synonym(s):
Bacterial cell lysis reagent
NACRES:
NA.56

form

powder

shipped in

wet ice

storage temp.

−20°C

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This Item
C4482CB0500C2978
vibrant-m

C1990

CelLytic Express

vibrant-m

C4482

CelLytic Y Cell Lysis Reagent

vibrant-m

CB0500

CelLytic B Plus Kit

vibrant-m

C2978

CelLytic M

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

Application

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.
CelLytic Express has been used as a component in lysis buffer for protein purification.
CelLytic Express is optimized for the lysis of E. coli strain BL21. However, it works well with other commons strains such as JM109. CelLytic Express may also be used on other similar bacterial cells. CelLytic Express has been tested with BL21 E. coli cells expressing histidine-tagged and FLAG fusion proteins. It should be compatib with affinity purification of other fusion proteins.

Features and Benefits

  • Efficient and non-denaturing in-culture protein extraction
  • Save time by eliminating cell harvest and clarification steps
  • Preserve biological activity with fewer manipulations
  • Compatible with affinity resins and magnetic beads
  • Ideal for Production Scale Extraction protocols

Preparation Note

CelLytic® Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic Express, and not by adding them to the dry powder.

Storage and Stability

This product ships on wet ice and storage at –20°C is recommended. CelLytic Express, as supplied, is stable for at least two years when stored properly.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
Celatom is a registered trademark of EP Minerals, LLC

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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