MilliporeSigma
All Photos(1)

Documents

CB0500

Sigma-Aldrich

CelLytic B Plus Kit

For bacterial lysis

Sign Into View Organizational & Contract Pricing

Synonym(s):
Lysis and protein extraction kit
EC Number:
NACRES:
NA.56

usage

 kit sufficient for 50 g fresh or frozen cell paste lysis.

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C1990CE0050C4482
vibrant-m

CB0500

CelLytic B Plus Kit

vibrant-m

C1990

CelLytic Express

vibrant-m

C4482

CelLytic Y Cell Lysis Reagent

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The CelLytic B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.

Application

This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chroma­tography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.

Features and Benefits

  • Lyse Gram positive and Gram negative bacteria
  • More efficient than sonication
  • Compatible with affinity purification
  • Isolate inclusion bodies for subsequent solubilization
  • Non-denaturing cell lysis preserves protein function

Other Notes

The CelLytic B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.

Legal Information

Benzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • B7435CelLytic B Cell Lysis Reagent, For bacterial cell lysis, standard strengthSDS

  • E1014Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solutionSDS

  • P8849Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solutionSDS

  • L3790Lysozyme from chicken egg white, 10 mg/mLSDS

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Benzonase® Nuclease ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Millipore

E1014

Benzonase® Nuclease

cOmplete™ Lysis-M storage temp.:room temp

Roche

04719956001

cOmplete Lysis-M

Millipore

Millipore

71285-M

BCA Protein Assay Kit

Custom DNA Oligos Design and Order Custom Oligos

OLIGO

Custom DNA Oligos

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service