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C9282

Sigma-Aldrich

Sodium cholate hydrate

suitable for cell culture, BioReagent

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Synonym(s):
3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid sodium salt, Cholalic acid sodium salt
Empirical Formula (Hill Notation):
C24H39NaO5 · xH2O
CAS Number:
Molecular Weight:
430.55 (anhydrous basis)
Beilstein:
3582354
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

biological source

bovine bile
ovine bile

description

anionic

product line

BioReagent

Assay

≥99.0% (HPLC)

form

powder

mol wt

micellar avg mol wt 900-1300

aggregation number

2-3

technique(s)

cell culture | mammalian: suitable

CMC

9-15 mM (20-25°C)

solubility

water: 100 mg/mL, clear, colorless to light yellow

HLB

18

SMILES string

O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C

InChI

1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1

InChI key

MUVVIYFKOVLQHL-RCVKHMDESA-M

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1 of 4

This Item
C125427029C6445
Sodium cholate hydrate suitable for cell culture, BioReagent

Sigma-Aldrich

C9282

Sodium cholate hydrate

-
Sodium cholate hydrate from bovine and/or ovine bile, ≥99%

Sigma-Aldrich

C1254

Sodium cholate hydrate

-
Sodium cholate hydrate ≥97.0% (dried material, NT)

Sigma-Aldrich

27029

Sodium cholate hydrate

-
Sodium cholate hydrate BioXtra, ≥99%

Sigma-Aldrich

C6445

Sodium cholate hydrate

Premium Grade
description

anionic

description

anionic

description

anionic

description

anionic

product line

BioReagent

product line

-

product line

-

product line

BioXtra

assay

≥99.0% (HPLC)

assay

≥99%

assay

≥97.0% (dried material, NT)

assay

≥99%

form

powder

form

powder

form

powder

form

powder

mol wt

micellar avg mol wt 900-1300

mol wt

micellar avg mol wt 900-1300

mol wt

micellar avg mol wt 900-1300

mol wt

micellar avg mol wt 900-1300

General description

Cholate is a primary bile acid produced from cholesterol in the liver. It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).

Application

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.
Sodium cholate hydrate has been used:
  • as a bile acid to induce bile acid-responsive (BEAR) transcription system,
  • as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
  • to test its sensitivity towards the bile acid sensor

Biochem/physiol Actions

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.

recommended

Product No.
Description
Pricing

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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P Fafournoux et al.
The Journal of biological chemistry, 264(9), 4805-4811 (1989-03-25)
In the liver, System A-mediated uptake of neutral amino acids may play a key role in metabolic control. Knowing the properties of the solubilized and reconstituted System A activity is important for future studies on the purification of the carrier
Mahendra Singh et al.
Cell biochemistry and biophysics, 48(1), 54-72 (2007-08-19)
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In recent years, using trigger-inducible mammalian gene switches to design sophisticated transcription-control networks has become standard practice in synthetic biology. These switches provide unprecedented precision, complexity and reliability when programming novel mammalian cell functions. Metabolite-responsive repressors of human-pathogenic bacteria are
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John Y L Chiang
Journal of lipid research, 50(10), 1955-1966 (2009-04-07)
Bile acids are physiological detergents that generate bile flow and facilitate intestinal absorption and transport of lipids, nutrients, and vitamins. Bile acids also are signaling molecules and inflammatory agents that rapidly activate nuclear receptors and cell signaling pathways that regulate

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