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SNL 76/7 Cell Line from mouse


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biological source

mouse esophagus


Mouse SIM strain embryonic fibroblast

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


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Cell Line Origin

Mouse SIM strain embryonic fibroblast

Cell Line Description

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

Culture Medium

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C.

Other Notes

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