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NS0 Cell Line from murine myeloma

Mouse myeloma, 85110503

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biological source

mouse skin

form

cell suspension

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse myeloma

Cell Line Description

NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1 ECACC catalogue no. 85011427). The cells are resistant to 10μM azaguanine, cells die in the presence of HAT medium. The NS0 cell line is the property of the Medical Research Council (MRC). ECACC is unable to supply this cell line to commercial entities unless a license has been first secured from LifeArc (formerly known as MRC Technology). Requests for commercial use of this cell line will be forwarded to LifeArc.

Application

Fusion partner
NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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Articles

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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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