Recommended Products
biological source
human breast
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human breast carcinoma
Cell Line Description
Tumorigenic in immunosuppressed mice and form colonies in semisolid medium. Neither oestrogen receptors nor endogenous viruses were detected.
Application
Hs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. It has also been used to study the changes in gene expression induced by insulin-like growth factor-binding protein (IGFBP-2).
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17
Culture Medium
DMEM + 2mM Glutamine + 10μg/ml Bovine Insulin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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