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C2C12 Cell Line from mouse

91031101, C3H muscle myoblast

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C2-C12 Cells

biological source

mouse muscle

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Mouse C3H muscle myoblast

Cell Line Description

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.


C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Culture Medium

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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