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DMS 53

95062823

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biological source

human lung

growth mode

Adherent

karyotype

Modal no. 60

morphology

Epithelial

products

Variety of hormones, high level of calcitonin

receptors

Bombesin (BN), Epidermal growth factor (EGF), Transforming growth factor (TGF-beta), acetylcholine receptor (AChR)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian lung small cell carcinoma

Cell Line Description

DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17β-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-β (TGF β) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.

Application

Human lung tumour model

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17

Culture Medium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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