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CRISPR13V

Sigma-Aldrich

CRISPR-Lenti Human HPRT1 Positive Control Transduction Particles

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NACRES:
NA.51

form

liquid

packaging

vial of 200 μL

concentration

1x106  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description

The Sigma CRISPR-LENTI HUMAN HPRT1 POSITIVE CONTROL TRANSDUCTION PARTICLES are a critical positive control to monitor transduction efficiency for lentiCRISPRs. This control is produced from the sequence-verified CRISPR lentiviral plasmid targeting human HPRT1. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for Sigma lenti CRISPRs. The Sigma lenti CRISPR positive control is a one-component system consisting of an EF1-alpha- driven Cas9, a U6-driven guide RNA plasmid targeting the human HPRT1 gene, with both Puromycin and GFP co-expressed with Cas9.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.

Application

Functional Genomics/Screening/Target Validation
  • Positive control, HPRT1, CRISPR lentivirus format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crispr.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lentiCRISPRs (virus format). Allows for validation of your system with the lentiCRISPR/Cas9 system. A positive result in a mis-match detection assay will indicate validation of your system.

Physical form

200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service