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CRISPR17

Sigma-Aldrich

CRISPR Activator Human Oct4 Positive Control Plasmid

gRNA Only Expression Plasmid

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Quality Level

form

liquid

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

Application

Functional Genomics/Target Validation
  • Epigenetic Modification
  • Transcriptional Activation

Features and Benefits

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

Components

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).

Linkage

To order U6-gRNA plasmids, please visit sigma.com/crisprs

Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.

Other Notes

Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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