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D0818

Sigma-Aldrich

Decalcifying Solution-Lite

aqueous solution, bp 208 °F

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NACRES:
NA.47

form

aqueous solution

Quality Level

100

shelf life

5 yr

color

light yellow

bp

208 °F

application(s)

hematology
histology

storage temp.

room temp

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This Item
HT903HT100465092E
Sigma-Aldrich

Sigma-Aldrich

D0818

Decalcifying Solution-Lite

Decolorizer Solution

Sigma-Aldrich

HT903

Decolorizer Solution

Gold Chloride Solution 200 mg/dL in deionized water

Sigma-Aldrich

HT1004

Gold Chloride Solution

Sigma-Aldrich

Sigma-Aldrich

65092E

Decolorizer Gram Solution

shelf life

5 yr

shelf life

-

shelf life

-

shelf life

-

color

light yellow

color

-

color

-

color

-

bp

208 °F

bp

-

bp

-

bp

-

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

storage temp.

no temp limit

General description

Decalcifying Solution-Lite is designed to be a universal and effective decalcifying agent.

Application

Decalcifying Solution-Lite is intended to be used for the decalcification of routine, immunohistochemical, and bone marrow core specimens.
It works equally well with all types of samples, including calvarial bone samples in an easy-to-handle time frame.

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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A Merchán-Pérez et al.
Histochemistry and cell biology, 112(2), 125-130 (1999-08-25)
An ascorbic acid decalcifying solution was applied to immuno- and affinohistochemical studies on the inner ear. Rat inner ears fixed in 4% paraformaldehyde in PBS or in 2% acetic acid in ethanol solutions were adequately decalcified in an ascorbic acid
Farhana Begum et al.
Journal of neuroscience methods, 187(1), 59-66 (2010-01-02)
Histological analysis of bone encased tissue is severely hampered by technical difficulties associated with sectioning calcified tissue. Cryosectioning of bone is possible but requires significant technical adaptation and expensive materials and is often time-consuming. Some decalcifying reagents in common use
P J Emans et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 80(3-4), 111-115 (2005-11-22)
Apoptosis is characterized by DNA strand breaks with a 3'-OH terminus, which are analyzed by terminal deoxy(d)-UTP nick end labeling (TUNEL). Proteinase K digestion is thought to be an essential step in the TUNEL procedure. The effects of decalcifying reagents
Roger W Byard et al.
Journal of forensic sciences, 55(5), 1356-1358 (2010-05-22)
To determine whether routine decalcification may reduce the amount of stainable iron that is visible on tissue sections, samples of liver and lung tissue with excessive iron stores were placed in three standard decalcifying solutions (i) formic acid [33%], formaldehyde
L F Machado-Silveiro et al.
International endodontic journal, 37(6), 365-369 (2004-06-10)
To measure the demineralization capability of 1 and 10% citric acid, 10% sodium citrate and 17% EDTA during immersions of 5, 10 and 15 min on root canal dentine. Crowns were sectioned from eight maxillary canines. The cementum was removed
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