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D4031

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

liquid, Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl₂, liquid, 0.1 μm filtered, suitable for cell culture

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Synonym(s):
DPBS
MDL number:
eCl@ss:
32129211

Quality Level

sterility

0.1 μm filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.036 g/L
glucose: 1.0 g/L

shipped in

ambient

storage temp.

2-8°C

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This Item
D1283D8662D5652
Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

500

form

liquid

form

liquid

form

liquid

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

impurities

-

sterility

0.1 μm filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

General description

Dulbecco′s phosphate buffered saline is a balanced salt solution, and a fully supplemented isotonic solution. It contains antibiotics and glucose and inorganic ions as energy sources. It is frequently used with primary cells where risk of contamination may be high.

Application

Dulbecco′s phosphate buffered saline has been used :
  • To study how ultrasound could enhance the delivery of anti inflammatory drug through cornea in corneal infections.
  • In the detection of microtubules in sludge worms.
  • For recovering bone marrow cells from the femur of green fluorescent protein (GFP) expressing mice.
  • To study the inhibitory effect of 3-methyladenine on autophagy, in normal rat kidney (NRK) cell expressing GFP-LC3 (autophagy marker).

Formula variant

Contains 50 mg/L streptomycin sulfate and 100 mg/L kanamycin monosulfate.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ultrasound-enhanced delivery of antibiotics and anti-inflammatory drugs into the eye
Nabili M, et al.
Ultrasound in Medicine & Biology, 39(4), 638-646 (2013)
Transplanted bone marrow cells do not provide new oocytes but rescue fertility in female mice following treatment with chemotherapeutic agents
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