Documents
F1283
Fetal Bovine Serum
Fetal Bovine Serum, USA origin, Ultra Low IgG
Recommended Products
biological source
bovine fetus
Quality Level
sterility
sterile-filtered
product line
FBS Select
composition
Bovine IgG, <5 μg/mL
Hemoglobin, ≤20 mg/dL
origin
USA origin
storage condition
avoid repeated freeze/thaw cycles
protect from light
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
pH
6.8-8.1
osmolality
260-330 mOsm/kg H2O
shipped in
dry ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | F4135 | 12103C | F2442 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| composition Bovine IgG, <5 μg/mL , Hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| storage condition avoid repeated freeze/thaw cycles, protect from light | storage condition - | storage condition - | storage condition - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable |
General description
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Packaging
Preparation Note
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Analysis Note
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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