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12106C

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

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Synonym(s):
HI FBS, HI FCS, HI Fetal Bovine Sera, HI Fetal Bovine Serum, FBS, FCS, sera, serum
MDL number:
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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This Item
12107CF0392F6765
Fetal Bovine Serum USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

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12106C

Fetal Bovine Serum

Fetal Bovine Serum USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture

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12107C

Fetal Bovine Serum

Fetal Bovine Serum USA origin, Dialyzed by ultrafiltration against 0.15 M NaCl, sterile-filtered, suitable for cell culture

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Fetal Bovine Serum USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

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F6765

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

composition

hemoglobin, ≤20 mg/dL

composition

hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

Application

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.

Preparation Note

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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E Anitua et al.
Experimental eye research, 119, 27-34 (2013-12-19)
We have developed and characterized a new type of plasma rich in growth factors (PRGF) derived eye-drop therapy for patients suffering from autoimmune diseases. To determine the concentration of several growth factors, proteins, immunoglobulins and complement activity of the heat-inactivated
Nand Lal Sharma et al.
The American journal of tropical medicine and hygiene, 72(6), 819-824 (2005-06-21)
Localized cutaneous leishmaniasis (LCL) in India is due mostly to Leishmania tropica. It is mainly endemic in the deserts of Rajasthan. Recently, Himachal Pradesh has been identified as a new endemic focus for the disease. In the last few years
Ruth M Risueño et al.
PloS one, 3(3), e1747-e1747 (2008-03-06)
How the T cell antigen receptor (TCR) discriminates between molecularly related peptide/Major Histocompatibility Complex (pMHC) ligands and converts this information into different possible signaling outcomes is still not understood. One current model proposes that strong pMHC ligands, but not weak
Li Zhou et al.
Genome biology, 9(7), R119-R119 (2008-07-31)
CD8+ NKT-like cells are naturally occurring but rare T cells that express both T cell and natural killer cell markers. These cells may play key roles in establishing tolerance to self-antigens; however, their mechanism of action and molecular profiles are
Silvia Goldoni et al.
The American journal of pathology, 173(3), 844-855 (2008-08-09)
Decorin, a member of the small leucine-rich proteoglycan gene family, down-regulates members of the ErbB receptor tyrosine kinase family and attenuates their signaling, leading to growth inhibition. We investigated the effects of decorin on the growth of ErbB2-overexpressing mammary carcinoma

Protocols

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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