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F5012

Sigma-Aldrich

Anti-Ferritin, Human antibody produced in rabbit

whole antiserum, liquid

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Synonym(s):
Anti-FHC, Anti-FTH, Anti-FTHL6, Anti-HFE5, Anti-PIG15, Anti-PLIF
MDL number:

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

15 mM sodium azide

species reactivity

human

technique(s)

Ouchterlony double diffusion: 1:16

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FTH1(2495)

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This Item
MABC602SAB1409042F6136
antibody form

whole antiserum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

human ... FTH1(2495)

Gene Information

human ... FTH1(2495)

Gene Information

human ... FTH1(2495)

Gene Information

horse ... Ftl(100051593)

technique(s)

Ouchterlony double diffusion: 1:16

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: 1 μg/mL

technique(s)

immunoelectrophoresis: suitable, indirect ELISA: 1:10,000

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General description

Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.

Immunogen

Ferritin isolated from normal human spleen

Application

  • Anti-Ferritin, Human antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blotting
  • immunocytochemical techniques

Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.

Preparation Note

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Scientific tests
Aldred EM, et al.
Pharmacology, 331-341 (2009)
Moon-Suhn Ryu et al.
The Journal of clinical investigation, 127(5), 1786-1797 (2017-04-05)
Developing erythrocytes take up exceptionally large amounts of iron, which must be transferred to mitochondria for incorporation into heme. This massive iron flux must be precisely controlled to permit the coordinated synthesis of heme and hemoglobin while avoiding the toxic
Zhao Wang et al.
International journal of hematology, 90(4), 501-505 (2009-08-06)
Elevated levels of serum ferritin and a low percentage of glycosylated ferritin have been reported in adult-onset Still's disease (AOSD) as well as in hemophagocytic lymphohistiocytosis (HLH). The objective of the current study was to investigate total ferritin levels and
Iron-Induced Retinal Damage
Handbook of nutrition, diet, and the eye, 619-626 (2014)
Pro-inflammatory activation of microglia in the brain of patients with sepsis
Zrzavy T, et al.
Neuropathology and Applied Neurobiology, 45(3), 278-290 (2019)

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