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F6522

Sigma-Aldrich

IgG2a−FITC Isotype Control from murine myeloma

clone UPC-10, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
IgG2a−Fluorescein isothiocyanate
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

clone

UPC-10, monoclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

flow cytometry: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
F6397M5409M5284
conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

UPC-10, monoclonal

clone

MOPC 21, monoclonal

clone

UPC-10, monoclonal

clone

MOPC 21, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

General description

IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

Specificity

Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.

Application

IgG2a FITC Isotype Control from murine myeloma has been used in dual-colour staining and in cell staining for flow cytometry.

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Three classes and four (sub) classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c
Bazin H, et al.
European Journal of Immunology, 4, 44-48 (1974)
T Nakamura et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(9), 5389-5394 (1998-06-06)
Activation of hepatic stellate (Ito) cells is a final common pathway of liver fibrosis. The findings presented in this paper indicate that expression of Na+/Ca2+ exchanger (NCX) emerges in rat hepatic stellate cells after activation in vitro during primary culture
Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1 * 1501 Is Regulated by Vitamin D
Ramagopalan SV, et al.
PLoS Genetics, 5(2), e1000369-e1000369 (2009)
Assessment of Contractility of Purified Smooth Muscle Cells Derived from Embryonic Stem Cells
Sinha S, et al.
Stem Cells, 24(7), 1678-1688 (2006)
J Schultz et al.
Cancer research, 60(23), 6663-6669 (2000-12-16)
Single-chain Fv antibody fragments from the CD20-specific murine monoclonal antibody B9E9 were genetically engineered as streptavidin fusions [single-chain Fv-streptavidin (scFvSA) fusion protein] for use in pretargeted radioimmunotherapy. The scFvSA constructs were expressed as soluble, tetrameric species in the periplasm of

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