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G1890

Sigma-Aldrich

Gelatin from porcine skin

powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

Porcine skin

sterility

sterile

type

Type A

product line

BioReagent

form

powder

mol wt

50—100 kDa

packaging

pkg of 1 kg
pkg of 100 g
pkg of 500 g

concentration

70—90% Biuret

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

surface coverage

100‑200 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear to hazy, faintly yellow

shipped in

ambient

storage temp.

room temp

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This Item
G9136G250048722
Gelatin from porcine skin medium gel strength, suitable for microbiology

Millipore

48722

Gelatin from porcine skin

sterility

sterile

sterility

γ-irradiated

sterility

-

sterility

non-sterile

form

powder

form

lyophilized powder

form

powder

form

powder

packaging

pkg of 1 kg, pkg of 100 g, pkg of 500 g

packaging

pkg of 10 mg

packaging

-

packaging

bottle of 100 g, bottle of 500 g

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

surface coverage

100‑200 μg/cm2

surface coverage

100‑200 μg/cm2

surface coverage

-

surface coverage

-

General description

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.

Application

Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Features and Benefits

  • Sterile gelatin sourced from porcine skin.
  • Free from proteases.
  • In the form of gelatin powder for convenient and easy handling.
  • Versatile for use in cell culture and electrophoresis techniques.
  • Provides adequate surface coverage for various applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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