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G9382

Sigma-Aldrich

Gelatin from bovine skin

gel strength ~225 g Bloom, Type B

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Synonym(s):
Bovine gelatin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.26

biological source

bovine skin

type

Type B

Assay

≥95% protein basis (biuret)

form

powder

technique(s)

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

solubility

H2O: soluble 50 mg/mL

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1 of 4

This Item
G9391G2500G2625
assay

≥95% protein basis (biuret)

assay

-

assay

70-90% (biuret)

assay

-

form

powder

form

powder

form

powder

form

powder

technique(s)

ELISA: suitable, cell culture | mammalian: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL

solubility

H2O: soluble 50 mg/mL, hazy to strongly hazy, faintly yellow to yellow

solubility

H2O: soluble 50 mg/mL

solubility

H2O: soluble 50 mg/mL

type

Type B

type

Type B

type

Type A

type

Type A

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from bovine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 225.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Comparison of γ-radiation and electron beam irradiation effects on gelatin.
Vieira FF, et al.
Radiation Physics and Chemistry, 63, 331-332 (2002)
K A van der Lee et al.
Journal of lipid research, 41(1), 41-47 (2000-01-11)
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