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G9136

Sigma-Aldrich

Gelatin from porcine skin

Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

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CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

Porcine skin

Quality Level

sterility

γ-irradiated

type

Type A

product line

BioXtra

form

lyophilized powder

packaging

pkg of 10 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

100‑200 μg/cm2

gel strength

~300 g Bloom

solubility

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

shipped in

ambient

storage temp.

room temp

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This Item
G1890G2625G2500
sterility

γ-irradiated

sterility

sterile

sterility

-

sterility

-

form

lyophilized powder

form

powder

form

powder

form

powder

packaging

pkg of 10 mg

packaging

pkg of 1 kg, pkg of 100 g, pkg of 500 g

packaging

-

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

surface coverage

100‑200 μg/cm2

surface coverage

100‑200 μg/cm2

surface coverage

-

surface coverage

-

Application

This product is recommended for use as a cell culture substratum at 100-200 μg/cm2. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization.

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