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G6795

Sigma-Aldrich

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

clone GSN24, purified from hybridoma cell culture

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Synonym(s):
Anti-GFP
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GSN24, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

concentration

~2 mg/mL

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C
2-8°C

target post-translational modification

unmodified

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G1546G6539G6670
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

GSN24, monoclonal

clone

GSN149, monoclonal

clone

GFP-20, monoclonal

clone

2.5D.17, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

technique(s)

indirect ELISA: suitable, western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins

technique(s)

dot blot: suitable, indirect ELISA: suitable, western blot: 1:2,000 using purified recombinant GFP preparation

technique(s)

immunohistochemistry: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of A549 cells

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General description

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Specificity

The antibody reacts specifically with GFP fusion proteins.

Application

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Biochem/physiol Actions

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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