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G7402

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

gold conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

aqueous glycerol suspension

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

technique(s)

dot blot: suitable
western blot: suitable

particle size

10 nm (colloidal gold)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
G7277A3937G7777
technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

gold conjugate

conjugate

gold conjugate

conjugate

alkaline phosphatase conjugate

conjugate

gold conjugate

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG.

Immunogen

Rabbit IgG purified from normal rabbit serum.

Application

Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Immunohistochemistry (1 paper)

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Ignaz Wessler et al.
PloS one, 11(6), e0156886-e0156886 (2016-06-11)
The worldwide use of neonicotinoid pesticides has caused concern on account of their involvement in the decline of bee populations, which are key pollinators in most ecosystems. Here we describe a role of non-neuronal acetylcholine (ACh) for breeding of Apis
G Kamp et al.
Reproduction (Cambridge, England), 133(1), 29-40 (2007-01-25)
Glycolysis is crucial for sperm functions (motility and fertilization), but how this pathway is regulated in spermatozoa is not clear. This prompted to study the location and the regulatory properties of 6-phosphofructokinase (PFK, EC 2.7.1.11), the most important element for
S Sinnarajah et al.
FEBS letters, 426(3), 377-380 (1998-05-26)
The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence
Andrea González et al.
The American journal of tropical medicine and hygiene, 92(5), 887-897 (2015-03-12)
Trypanosoma cruzi calreticulin (TcCRT), a 47-kDa chaperone, translocates from the endoplasmic reticulum to the area of flagellum emergence. There, it binds to complement components C1 and mannan-binding lectin (MBL), thus acting as a main virulence factor, and inhibits the classical
R Fabian-Fine et al.
The Journal of comparative neurology, 420(2), 195-210 (2001-02-07)
The mechanosensory neurons of arachnids receive diverse synaptic inputs in the periphery. The function of most of these synapses, however, is unknown. We have carried out detailed electron microscopic investigations of the peripheral synapses at sensory neurons in the compound

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