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G7277

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)−Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

gold conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

aqueous glycerol suspension

availability

not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)

technique(s)

dot blot: suitable
western blot: suitable

particle size

5 nm (colloidal gold)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
G7402G5402G7777
biological source

goat

biological source

goat

biological source

rabbit

biological source

goat

conjugate

gold conjugate

conjugate

gold conjugate

conjugate

gold conjugate

conjugate

gold conjugate

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable

technique(s)

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

aqueous glycerol suspension

form

aqueous glycerol suspension

form

aqueous glycerol suspension

form

aqueous glycerol suspension

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General description

Goat polyclonal anti-Rabbit IgG (whole molecule)−Gold antibody binds all rabbit IgGs. It has no observed reactivity with human serum proteins.
Immunoglobulins (Igs) have two heavy (H) and two light (L) chains, held together by disulphide linkages. They belong to the immunoglobulin super-family. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The light chain has one variable and constant region. IgGs are subclassified into IgG1, IgG2, IgG3 and IgG4. The IgG gene is localised on rabbit chromosome 20. Gene conversion events are observed in rabbit IgG gene.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.

Application

Anti-Rabbit IgG (whole molecule)−Gold antibody produced in goat has been used in electron microscopic analysis of exosomes in glioma cells, clathrin proteins and retinal tissues.
Goat polyclonal anti-Rabbit IgG (whole molecule)−Gold antibody may be used as a primary or secondary antibody that enables the labeling of rabbit IgG for detection by electron microscopy.

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). Fc regions of IgG antibody have enormous therapeutic potential and are exploited for the development of therapeutic antibodies. IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Safiye Cavdar et al.
Neuroscience bulletin, 30(3), 451-460 (2014-02-15)
The inhibitory sources in the thalamic nuclei are local interneurons and neurons of the thalamic reticular nucleus. Studies of models of absence epilepsy have shown that the seizures are associated with an excess of inhibitory neurotransmission in the thalamus. In
Simara Price et al.
Microbiology (Reading, England), 160(Pt 10), 2148-2156 (2014-07-11)
Collagen molecules are structural in nature and primarily found in eukaryotic, multicellular organisms. Recently, a collagen-like protein, TrpA, was identified and characterized in the marine cyanobacterium Trichodesmium erythraeum IMS 101, and it was shown to be involved in maintaining the
Localization of alphaA-Crystallin in Rat Retinal Muller Glial Cells and Photoreceptors
Zayas-Santiago A, et al.
Microscopy and Microanalysis, 24(5), 545-552 (2018)
Javier Figueroa et al.
Cancer research, 77(21), 5808-5819 (2017-09-01)
Tumor-stromal communications impact tumorigenesis in ways that are incompletely understood. Here, we show that glioma-associated human mesenchymal stem cells (GA-hMSC), a newly identified stromal component of glioblastoma, release exosomes that increase the proliferation and clonogenicity of tumor-initiating glioma stem-like cells
Accuracy and coverage assessment of Oryctolagus cuniculus (rabbit) genes encoding immunoglobulins in the whole genome sequence assembly (OryCun2. 0) and localization of the IGH locus to chromosome 20
Gertz EM, et al.
Immunogenetics, 65(10), 749-762 (2013)

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