GE17-0409-01
HiTrap® Chelating High Performance
Cytiva 17-0409-01, pack of 1 × 5 mL
Recommended Products
ligand
iminodiacetic acid
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-0409-01
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14
working range
3-13
Related Categories
General description
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
- Conveniently charge with your metal of choice.
- Iminidoacetic acid coupled to Sepharose™ High Performance.
- Purifying Proteins with exposed histidine groups via metal ion complex formation.
- Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.
- Packed with Chelating Sepharose™ High Performance
- Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System
Storage and Stability
Analysis Note
Other Notes
Legal Information
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Warning
hcodes
Certificates of Analysis (COA)
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Articles
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Protocols
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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