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GE17-1179-01

Resource Q

Cytiva 17-1179-01, pack of 6 mL

Synonym(s):

Resource Q Column

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About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

ligand

quaternary ammonium

description

Ion Exchanger Type (value)

packaging

pack of 6 mL

manufacturer/tradename

Cytiva 17-1179-01

parameter

87 psi

bed size

16 mm × 30 mm

bed volume

6 mL

matrix

polystyrene/divinylbenzene

avg. part. size

15 μm

particle size

15-15 μm

cleaning

1-14

working range

2-12

suitability

suitable for bioprocess medium

Related Categories

General description

RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Application

Automated pilot scale purification of synthetic oligonucleotides; AKTAexplorer Summary
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.

Features and Benefits

  • SOURCE 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • RESOURCE prepacked columns for fast separation. Separation times using 1 mL RESOURCE columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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Protocols

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

This page discusses how to do a purification with SOURCE™ media from Cytiva.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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