GE17-6002-33
HiTrap® IEX Selection Kit
Cytiva 17-6002-33
Synonym(s):
IEX Selection Kit
About This Item
Recommended Products
ligand
quaternary amine
description
Ion Exchanger Type (value)
packaging
pkg of 7 ea
manufacturer/tradename
Cytiva 17-6002-33
parameter
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
Related Categories
General description
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.
Features and Benefits
- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1 mL HiTrap® columns.
- Offers a fast, easy and convenient way screen for the optimal media for a given application .
- Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.
Storage and Stability
Analysis Note
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