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H0654

Sigma-Aldrich

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H2O

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Synonym(s):
Hygromycin B, Hygrovetine
Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
Molecular Weight:
527.52
Beilstein:
6755837
MDL number:
PubChem Substance ID:
NACRES:
NA.85

biological source

Streptomyces hygroscopicus

Quality Level

sterility

0.22 μm filtered

concentration

≥60% (HPLC)
45-60 mg/mL in H2O

color

brown-yellow
light orange to dark orange
light yellow to dark yellow

antibiotic activity spectrum

viruses

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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This Item
SBR00039H5527H9773
sterility

0.22 μm filtered

sterility

-

sterility

γ-irradiated

sterility

-

concentration

≥60% (HPLC), 45-60 mg/mL in H2O

concentration

50 mg/mL in water (from Streptomyces hygroscopicus)

concentration

≥60% (HPLC), 45-60 mg/mL in H2O

concentration

≥60% (HPLC)

color

brown-yellow, light orange to dark orange, light yellow to dark yellow

color

-

color

yellow-brown to orange

color

faintly brown to brown, white to beige

antibiotic activity spectrum

viruses

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, fungi, viruses

antibiotic activity spectrum

viruses

antibiotic activity spectrum

fungi

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

General description

Chemical structure: aminoglycoside

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Caution

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Preparation Note

This product is a solution that comes =60% (HPAE) and 45-60 mg/mL in H2O.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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