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H2519

Sigma-Aldrich

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Synonym(s):

Heparin lyase I, Heparinase

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About This Item

CAS Number:
9025-39-2
Enzyme Commission number:
4.2.2.7 (BRENDA, IUBMB)
MDL number:
MFCD00131317
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

form

lyophilized solid

specific activity

≥200 units/mg protein (enzyme + BSA)

mol wt

42.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Heparinase I from Flavobacterium heparinum has been used:
  • in the digestion of plasma samples[1][2]
  • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia[3]
  • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells[4]

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin.[5] This results in small oligosaccharide fragments[6] . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.[6]

Unit Definition

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000429785
0000429785
0000407596
0000407595
0000407595

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Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro
Yan Y, et al.
Journal of Microbiological Methods, 61(1), 127-130 (2005)
M E Casey et al.
Anaesthesia, 68(1), 91-96 (2012-10-16)
Paediatric patients who require anticoagulation with therapeutic doses of low-molecular weight heparin are at risk of having a residual anticoagulant effect at the time of surgery, even if managed according to current peri-operative guidelines. Testing for residual effect is not
HeparinHeparin - A Century of Progress, 273-273 (2012)
Wataru Hashimoto et al.
Biochemistry, 53(4), 777-786 (2014-01-21)
Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
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