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H2519

Sigma-Aldrich

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

Synonym(s):

Heparin lyase I, Heparinase

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About This Item

CAS Number:
9025-39-2
Enzyme Commission number:
4.2.2.7 (BRENDA, IUBMB)
MDL number:
MFCD00131317
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

conjugate

conjugate (Glucosaminoglycan)

Quality Level

200

form

lyophilized solid

specific activity

≥200 units/mg protein (enzyme + BSA)

mol wt

42.8 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Heparinase I from Flavobacterium heparinum has been used:
  • in the digestion of plasma samples[1][2]
  • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia[3]
  • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells[4]

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin.[5] This results in small oligosaccharide fragments[6] . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.[6]

Unit Definition

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000429785
0000429785
0000407596
0000407595
0000407595

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Dong-Ja Kim et al.
The Journal of molecular diagnostics : JMD, 14(1), 71-80 (2011-12-14)
Circulating microRNAs (miRNAs) have emerged as candidate biomarkers of various diseases and conditions including malignancy and pregnancy. This approach requires sensitive and accurate quantitation of miRNA concentrations in body fluids. Herein we report that enzyme-based miRNA quantitation, which is currently
Udayanath Aich et al.
Analytical chemistry, 83(20), 7815-7822 (2011-08-26)
Heparin and the low molecular weight heparins are extensively used as medicinal products to prevent and treat the formation of venous and arterial thrombi. In early 2008, administration of some heparin lots was associated with the advent of severe adverse
Avery Davis Bell et al.
Nature, 583(7815), 259-264 (2020-06-05)
Meiosis, although essential for reproduction, is also variable and error-prone: rates of chromosome crossover vary among gametes, between the sexes, and among humans of the same sex, and chromosome missegregation leads to abnormal chromosome numbers (aneuploidy)1-8. To study diverse meiotic
Wataru Hashimoto et al.
Biochemistry, 53(4), 777-786 (2014-01-21)
Pedobacter heparinus (formerly known as Flavobacterium heparinum) is a typical glycosaminoglycan-degrading bacterium that produces three heparin lyases, Hep I, Hep II, and Hep III, which act on heparins with 1,4-glycoside bonds between uronate and amino sugar residues. Being different from
Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
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