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H4777

Sigma-Aldrich

Heparan sulfate proteoglycan

≥400 μg/mL glycosaminoglycan

Synonym(s):
HSPG
MDL number:
NACRES:
NA.75

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Quality Level

description

For cell culture use.
Isolated from basement membrane of Engelbreth-Holm-Swarm mouse sarcoma.
Solution in 50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.4, containing ≥400 μg protein per ml.

form

solution

packaging

pkg of 0.1 mg

concentration

≥400 μg/mL glycosaminoglycan

technique(s)

cell culture | mammalian: suitable

impurities

≥100 μg/mL Uronic acid

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Hspg2(15530)

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This Item
L2020I4161C0543
form

solution

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

packaging

pkg of 0.1 mg

packaging

pkg of 1 mg

packaging

pkg of 5X20 μg, pkg of 20 μg

packaging

glass bottle of 1 vial

concentration

≥400 μg/mL glycosaminoglycan

concentration

1-2 mg/mL in Tris-buffered saline

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

≥100 μg/mL Uronic acid

impurities

-

impurities

≤0.200ng/ml

impurities

-

Application

Heparan Sulfate promotes the attachment of cells in culture and induces high affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.

Biochem/physiol Actions

This is a multifunctional molecule. As a component of the extracellular matrix that binds to fibroblast growth factors, vascular endothelial growth factor and vascular endothelial growth factor receptors. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases.

Components

Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.

Caution

It is recommended to store this product at 20°C, where it will retain its function for 3 years

Preparation Note

This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Glycosaminoglycans and Proteoglycans

There are five identified glycosaminoglycan chains (see Figure 1): Hyaluronan is not sulfated, but the other glycosaminoglycan chains contain sulfate substituents at various positions of the chain.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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