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H8509

Sigma-Aldrich

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

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Synonym(s):
(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type V

form

lyophilized powder

specific activity

250-750 units/mg protein

purified by

chromatography

foreign activity

lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%

storage temp.

−20°C

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This Item
H9408L3888G6880
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

purified by

chromatography

purified by

chromatography

purified by

-

purified by

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

lactate dehydrogenase ≤0.05%, malate dehydrogenase ≤0.1%

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

foreign activity

Malic dehydrogenase <0.5% of base activity

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Unit Definition

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Succinic semialdehyde reductase (SSAR) is an important enzyme involved in γ-aminobutyrate (GABA) metabolism. By converting succinic semialdehyde (SSA) to γ-hydroxybutyrate (GHB), the SSAR facilitates an alternative pathway for GABA degradation. In this study, we identified SSARs from Geobacter sulfurreducens and
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