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H9037

Sigma-Aldrich

Anti-HA−TRITC antibody, Mouse monoclonal

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

TRITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

storage condition

protect from light

concentration

~1 mg/mL

technique(s)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

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1 of 4

This Item
B9183H3663H6908
vibrant-m

H3663

Anti-HA antibody, Mouse monoclonal

vibrant-m

H6908

Anti-HA antibody produced in rabbit

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

conjugate

TRITC conjugate

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

unconjugated

clone

HA-7, monoclonal

clone

HA-7, monoclonal

clone

HA-7, monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

technique(s)

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

technique(s)

immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL

technique(s)

immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts

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General description

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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