MilliporeSigma
All Photos(1)

Documents

HT5011

Sigma-Aldrich

Formalin solution, neutral buffered, 10%

case of 48 × 15 mL, histological tissue fixative

Sign Into View Organizational & Contract Pricing

Synonym(s):
Formaldehyde, Methanal
MDL number:
PubChem Substance ID:
NACRES:
NA.47

shelf life

Expiry date on the label.

packaging

case of 48 × 15 mL

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

Application

10% neutral buffered formalin (NBF) is a popular general purpose histological fixative. It has been used to fix tissues, protein complexes and tumor samples.
Popular general purpose histological fixative.

Packaging

Case containers are polypropylene with white leak-proof lids and are partially prefilled with 10% NBF.

Physical form

Neutral buffered solution

Storage and Stability

Store tightly closed at room temperature. Do not freeze.

related product

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

185.0 °F

flash_point_c

85 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Formaldehyde solution for molecular biology, 36.5-38% in H2O

Sigma-Aldrich

F8775

Formaldehyde solution

Formaldehyde solution for molecular biology, BioReagent, ≥36.0% in H2O (T)

Sigma-Aldrich

47608

Formaldehyde solution

Mangiferin induces islet regeneration in aged mice through regulating p16INK4a.
Wang H, et al.
International Journal of Molecular Medicine, 41(6), 3231-3242 (2018)
Biological characterization of a Stable Effector Functionless (SEFL) monoclonal antibody scaffold in vitro.
Liu L, et al.
The Journal of Biological Chemistry, 292(5), 1876-1883 (2017)
Nuclear GSK3β induces DNA double-strand break repair by phosphorylating 53BP1 in glioblastoma.
Yang Y, et al.
International Journal of Oncology (2018)
Rune M Pedersen et al.
Frontiers in cellular and infection microbiology, 8, 16-16 (2018-02-17)
Most bacterial infections initiate at the mucosal epithelium lining the gastrointestinal, respiratory, and urogenital tracts. At these sites, bacterial pathogens must adhere and increase in numbers to effectively breach the outer barrier and invade the host. If the bacterium succeeds
Parco M Siu et al.
BMC musculoskeletal disorders, 12, 58-58 (2011-03-10)
The molecular mechanism initiating deep pressure ulcer remains to be elucidated. The present study tested the hypothesis that the ubiquitin proteasome system is involved in the signalling mechanism in pressure-induced deep tissue injury. Adult Sprague Dawley rats were subjected to

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service