type
Type IV
Quality Level
form
buffered aqueous glycerol solution
quality
≤ 0.2% of main activity is obtained with NAD as cofactor.
specific activity
3-20 units/mg protein
composition
Protein biuret
shipped in
wet ice
storage temp.
2-8°C
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Application
Isocitric dehydrogenase (IDH) has been used:
- for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
- as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
- in isocitrate assay
Biochem/physiol Actions
Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH1, IDH2, and IDH3.
Unit Definition
One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.
Physical form
Solution in 50% glycerol in EDTA buffer salts, pH 6.0
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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