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I8640

Sigma-Aldrich

IgG from human serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

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Synonym(s):
Human IgG
MDL number:
NACRES:
NA.46

conjugate

unconjugated

Quality Level

grade

technical grade

Assay

≥80% (SDS-PAGE)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
I4506I2511I8260
Sigma-Aldrich

Sigma-Aldrich

I8640

IgG from human serum

IgG from human serum reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Sigma-Aldrich

I4506

IgG from human serum

IgG from human serum reagent grade, ≥95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I2511

IgG from human serum

IgM from human serum reagent grade, ~95% (HPLC), buffered aqueous solution

Sigma-Aldrich

I8260

IgM from human serum

form

buffered aqueous solution

form

essentially salt-free, lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

grade

technical grade

grade

reagent grade

grade

reagent grade

grade

reagent grade

assay

≥80% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (HPLC)

assay

~95% (HPLC)

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis among many others. IgG from human serum can be used as an economical alternative for reagent grade immunoglobulins where high purity is not essential. It can also be used in immunoelectrophoresis. The product is reactive in humans.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Human IgG is purified from normal human serum by fractionation.

Application

Purified human IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Technical grade human IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. IgG from human serum was used as ELISA standard in various studies. A concentration range of 200 μg/ml to 1 mg/ml of human IgG was used for blocking in flow cytometry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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