M2520
Medium 199
HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
About This Item
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM
shipped in
ambient
storage temp.
2-8°C
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General description
Application
- maintain the cumulus-oocyte complex (COC) before maturation and for culturing of these oocytes for in vitro maturation
- to rinse the aspiration line during the collection of the ovum from German Holstein cows
- to culture cardiac endothelial cells from rats
Quantity
Reconstitution
also commonly purchased with this product
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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