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M5017

Sigma-Aldrich

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

product name

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Related Content

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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