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G6148

Sigma-Aldrich

Glasgow Minimum Essential Medium

With L-glutamine, without tryptose phosphate broth and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

BHK medium, GMEM

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About This Item

MDL number:
MFCD00243118
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

Glasgow Minimum Essential Medium is suitable for the study of genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used:
  • in toxin entry assays
  • for 3 H-palmitate labeling
  • to culture embryonic stem cells

Quantity

Formulated to contain 12.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.75 g/L sodium bicarbonate and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

also commonly purchased with this product

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supplement

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Description
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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Keiichi Ito et al.
Genes & development, 35(9-10), 729-748 (2021-04-24)
The MED1 subunit has been shown to mediate ligand-dependent binding of the Mediator coactivator complex to multiple nuclear receptors, including the adipogenic PPARγ, and to play an essential role in ectopic PPARγ-induced adipogenesis of mouse embryonic fibroblasts. However, the precise
Tatsushi Miyazaki et al.
PloS one, 8(8), e71645-e71645 (2013-08-14)
Tcl1 is highly expressed in embryonic stem (ES) cells, but its expression rapidly decreases following differentiation. To assess Tcl1's roles in ES cells, we generated Tcl1-deficient and -overexpressing mouse ES cell lines. We found that Tcl1 was neither essential nor
Naiara Clemente Tavares et al.
Frontiers in microbiology, 11, 963-963 (2020-07-01)
Schistosomiasis is a neglected tropical disease (NTD) caused by helminthes from the Schistosoma genus. This NTD can cause systemic symptoms induced by the deposition of parasite eggs in the host liver, promoting severe complications. Functional studies to increase knowledge about
Wander de Jesus Jeremias et al.
PloS one, 12(6), e0178829-e0178829 (2017-06-18)
Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the
Edward C Hsiao et al.
PloS one, 3(7), e2532-e2532 (2008-07-04)
Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter
View All Related Papers

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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