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M7449

Sigma-Aldrich

MG-132, Ready Made Solution

≥90% (HPLC)

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EC Number:
NACRES:
NA.32

Quality Level

Assay

≥90% (HPLC)

form

DMSO solution

quality

useful for proteasome inhibition

concentration

10 mM in DMSO

shipped in

dry ice

storage temp.

−20°C

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This Item
M5353B7216474788
form

DMSO solution

form

liquid

form

liquid

form

liquid

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

quality

useful for proteasome inhibition

quality

-

quality

-

quality

-

Amino Acid Sequence

Z-Leu-Leu-Leu-al

General description

MG132 is a potent proteasome inhibitor that also inhibits calpains and lysosomal cathepsins. One of the first proteasome inhibitors to be developed, MG-132 is widely used in proteasome inhibition studies because of its high potency, high selectivity, and rapid reversibility. MG132 mechanistically acts as a proteasome inhibitor by hemiacetal formation with hydroxyl groups of active site threnonine residues.

Application

MG-132, Ready Made Solution has been used to inhibit the proteasome in various systems, including:
  • hTERT-RPE1 and HeLa cells: Osorio-Zambrano, W.F. et al., PLoS One, 10(12), e0144434 (2015)
  • FaDu human hypopharynx cancer cells: Kim, S.-Y. et al., Autophagy, 14(3), 385-403 (2018)
  • HEK293 cells: Chen, Q. et al., Cell Reports, 22(11), 2860-2872 (2018).
  • Neonate rat cardiomyocytes: Turkieh, A. et al., Cell Death Dis., 10(8), 608 (2019)
  • Adult neural stem cells: Morrow, C.S. et al., Cell Stem Cell, 26(4), 558-568.e9 (2020).

Biochem/physiol Actions

MG132, also known as carbobenzoxy-Leu-Leu-leucinal, is a peptide aldehyde which plays a vital role in the inhibition of proteolytic activity of the 26S proteasome complex. MG132 retards the growth of HeLa cells by promoting cell cycle arrest and apoptosis. Treatment of HepG2 and HeLa cells with MG-132 triggers increased expression of MCPIP1 mRNA, which in turn increases MCPIP1 protein concentration. MCPIP1 is implicated in negative regulation of macrophage activation and differentiation of several cell types, including pre-adipocytes, neuroprogenitor cells and osteoclast precursors. MG-132 stimulates dopamine reduction and nigral dopaminergic degeneration in both cell culture and animal models of Parkinson′s disease.
Potent, membrane-permeable proteasome inhibitor. Induces neurite outgrowth in PC12 cells at 10 μM. Blocks cleavage of poly(ADP-ribose) polymerase and apoptosis in thymocytes. Proteasome inhibition induces accumulation of heat shock protein mRNA, activation of heat-shock proteins, and enhanced thermotolerance in various cell types: however, it also activates JNK-1, which initiates apoptosis in response to cell stress.

Features and Benefits

  • Ready-to-use solution in DMSO
  • Purity tested by HPLC

Physical form

MG-132, Ready Made Solution is supplied as a 10 mM (1 mg/210 μL) 0.2 μm-filtered solution in DMSO.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


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Customers Also Viewed

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Proteasome inhibitor MG-132 induces MCPIP1 expression.
Skalniak L
FEBS Journal, 280(11), 2665-2674 (2013)
Loss-of-function mutations in the ATP13A2/PARK9 gene cause complicated hereditary spastic paraplegia (SPG78).
Estrada-Cuzcano A
Brain, 140(Pt 2), 287-305 (2017)
Proteasome inhibitor MG-132 induces dopaminergic degeneration in cell culture and animal models.
Sun F
Neurotoxicology, 27(5), 807-815 (2006)
RhoA Activation Sensitizes Cells to Proteotoxic Stimuli by Abrogating the HSF1-Dependent Heat Shock Response.
Meijering RA
PLoS ONE, 10(7), e0133553-e0133553 (2015)
The effect of MG132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH.
Han YH
Oncology Reports, 22(1), 215-221 (2009)

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