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MAK055

Sigma-Aldrich

AST Activity Assay Kit

sufficient for 100 colorimetric tests

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Synonym(s):
Aspartate Aminotransferase Activity Assay Kit, Glutamate-Oxaloacetate Transaminase Activity Assay Kit, SGOT Activity Assay Kit, Serum Glutamate-Oxaloacetate Transaminase Activity Assay Kit
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

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vibrant-m

MAK055

AST Activity Assay Kit

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MAK114

Glyoxalase I Activity Assay Kit

vibrant-m

MAK052

ALT Activity Assay

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric, fluorometric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 tests (UV)

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 500 colorimetric tests

relevant disease(s)

gastrointestinal diseases; cardiovascular diseases

relevant disease(s)

cancer

relevant disease(s)

gastrointestinal diseases

relevant disease(s)

hematological disorder; cancer

General description

Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.

Application

AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum

Principle

The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 μmole of glutamate per minute at pH 8.0 at 37 °C.

Replaced by

pictograms

CorrosionHealth hazard

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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