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MAK124

Sigma-Aldrich

BCG Albumin Assay Kit

sufficient for 250 colorimetric tests

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Synonym(s):
Bromocresol Green Albumin Assay
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ALB(213)
mouse ... ALB(11657)
rat ... ALB(24186)

Related Categories

General description

Albumin is the most abundant plasma protein in humans. It accounts for ∼60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, hematin, calcium, and magnesium. It has antioxidant and anticoagulant effects, acts as a carrier for nutritional factors and drugs, and is an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration.

Application


  • LncRNA ZEB1-AS1 inhibits renal fibrosis in diabetic nephropathy by regulating the miR-217/MAFB axis.: This research utilizes the BCG Albumin Assay Kit to quantitatively analyze albumin levels in studies of diabetic nephropathy. The results demonstrate the regulatory role of lncRNA on miR-217/MAFB axis, contributing significantly to the understanding and potential treatments of kidney fibrosis in diabetic patients (Song et al., 2019).

  • Albumin depletion of human plasma also removes low abundance proteins including the cytokines.: This study employs the BCG Albumin Assay Kit to investigate the effects of albumin depletion in human plasma, specifically its impact on the presence of low abundance proteins such as cytokines. The findings are crucial for the refinement of proteomic analyses and understanding protein interactions in biological samples (Granger et al., 2005).


Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of hemoglobin in serum, plasma, urine, and other biological samples.

Principle

The BCG (Bromocresol Green) albumin assay kit is designed to measure albumin directly without any pretreatment of samples. The optimized formulation substantially reduces interference by other substances (lipids/other proteins) in the raw samples. It may also be used to measure effects of drugs and other compounds on albumin metabolism. The procedure involves addition of a single working reagent and a 5 minute incubation. The optimized formulation has greatly enhanced reagent and signal stability. The kit utilizes bromocresol green, which forms a colored complex specifically with albumin. The intensity of the color, measured at 620 nm, is directly proportional to the albumin concentration in the sample.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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