MAK095
Aspartate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
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usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders
storage temp.
−20°C
General description
Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate.[1] In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.[2][3]
Application
Suitability
The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.
Principle
Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).
replaced by
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Essentials of medical biochemistry: with clinical cases.
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ESSENTIALS OF MEDICINAL CHEMISTRY, 2011 null
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