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MAK080

Sigma-Aldrich

Creatinine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

genitourinary disorders; neurological disorders

storage temp.

−20°C

General description

Creatinine is generated from creatine by nonenzymatic dehydration. Creatinine is produced at a constant rate and is excreted from the body through kidney glomerular filtration. Decreased kidney function can effect the rate at which creatinine is filtered by the kidneys and can be used as a measure of kidney function. Decreased kidney function can result in increased serum creatinine levels due to the inability to clear creatinine through urine excretion. Creatinine levels can be affected by changes in muscle mass, pregnancy, or the use of angiotensin inhibitors or angiotensin receptor antagonists.

Application

Creatinine Assay Kit has been used to measure the creatinine concentration in samples.

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, Creatinine concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535em/λ = 587 nm) product, proportional to the creatinine present.

replaced by

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Creatinine United States Pharmacopeia (USP) Reference Standard

USP

1150353

Creatinine

Qianjing Xia et al.
JCI insight, 5(18) (2020-08-19)
BACKGROUNDControl of the tuberculosis (TB) pandemic remains hindered in part by a lack of simple and accurate measures of treatment efficacy, as current gold standard markers rely on sputum-based assays that are slow and challenging to implement. However, previous work
Creasensor: SIMPLE technology for creatinine detection in plasma.
Dal DF, et al.
Analytica Chimica Acta, 1000, 191-198 (2018)
Taraneh Taghavi et al.
Addiction (Abingdon, England), 113(11), 2087-2096 (2018-06-20)
Smokers can regulate their nicotine intake by altering the number of cigarettes smoked per day (CPD) and their smoking intensity. The current study aimed to compare the utility of self-reported CPD, total nicotine equivalents (TNE) and urinary cotinine to estimate
Sarah S Dermody et al.
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco, 22(2), 256-263 (2018-12-19)
Alcohol may influence the nicotine metabolite ratio (NMR), an index of the rate of nicotine metabolism that is associated smoking level and lapses. We examined if NMR changes during alcohol use disorder (AUD) treatment and how changes in NMR relate
Tracy Nelson et al.
Journal of the American Society of Nephrology : JASN, 30(9), 1559-1572 (2019-08-14)
We recently showed the transcription factor Early B cell factor 1 (EBF1) is essential for the last stages of metanephric development, and that mice globally deficient in EBF1 display impaired maturation of peripheral glomeruli. EBF1 is present within multiple glomerular

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