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MAK084

Sigma-Aldrich

Glucose Uptake Fluorometric Assay Kit

sufficient for 100 fluorometric tests

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NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

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MAK156

Fatty Acid Uptake Kit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

fluorometric

detection method

colorimetric, fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

endocrinological disorders, diabetes

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

relevant disease(s)

-

General description

The new Glucose Uptake Assay Kit, MAK489, is now available! Glucose is the primary source of energy for most cells.Transport of glucose across the plasma membrane is the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.

Application

Glucose Uptake Fluorometric Assay Kit has been used to measure the amount of glucose uptake by monocytic myeloid-derived suppressor cells (M-MDSCs).

Suitability

Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.

Principle

The Glucose Uptake Fluorometric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which reacts with the probe to generate a fluorometric (λex = 535/λem = 587 nm) product, proportional to the 2-DG taken up by the cell.

related product

replaced by

Product No.
Description
Pricing

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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Yang Zhao et al.
Blood, 131(14), 1587-1599 (2018-02-22)
Monocytes and macrophages play a key role in defending pathogens, removing the dead cells or cell debris, and wound healing. The mammalian target of rapamycin (mTOR) inhibitor rapamycin (RPM) is widely used in clinics to treat patients with organ transplantation
Chapter Twenty-Two Mechanisms and Methods in Glucose Metabolism and Cell Death.
Zhao Y, et al.
Methods in Enzymology, 442, 439-457 (2008)
Norio Yamamoto et al.
Current protocols in pharmacology, Chapter 12, Unit 12-Unit 12 (2011-12-08)
Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes
Norio Yamamoto et al.
Analytical biochemistry, 351(1), 139-145 (2006-01-31)
A nonradioisotope, 96-well-microplate assay to evaluate glucose uptake activity in cultured cells has been developed. 2-Deoxyglucose (2DG) was detected by measuring a potent fluorophore, resorufin, generated after incubation with a single assay solution containing hexokinase, adenosine 5'-triphosphate, glucose 6-phosphate dehydrogenase
Norio Yamamoto et al.
Analytical biochemistry, 404(2), 238-240 (2010-05-25)
Previously, we developed a microplate assay to quantitate 2-deoxyglucose (2DG) and 2-deoxyglucose-6-phosphate in samples for in vitro and in vivo use. In this assay system, four different reaction mixtures were used, and the difference in the reactivity of the two

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