MAK562
Branched Chain Amino Acid Kit
sufficient for 100 colorimetric tests
Synonym(s):
BCAA Assay Kit, BCAA Kit
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About This Item
UNSPSC Code:
41116104
NACRES:
NA.28
Recommended Products
usage
sufficient for 100 colorimetric tests
Quality Level
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders; gastrointestinal diseases
storage temp.
−20°C
General description
Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver1. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual.2
In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD+ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.
In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD+ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.
Application
BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.
Features and Benefits
Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Suitability
Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 3
Certificates of Analysis (COA)
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Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo
Livesey G, et al.
The Biochemical Journal, 188, 705-713 (1980)
P W Lemon et al.
Medicine and science in sports and exercise, 13(3), 141-149 (1981-01-01)
At rest, especially during starvation, protein (via hepatic gluconeogenesis and/or direct oxidation) contributes significantly to the body's total metabolic requirement. During exercise, carbohydrate (CHO) and free fatty acids (FFA) are generally regarded as the major energy sources. Although protein has
A simple enzymatic fluorimetric method for the determination of branched-chain L-amino acids in microlitre volumes of plasma
Gleeson M, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 166, 163-169 (1987)
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