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MTOX1071

Sigma-Aldrich

Kidney PTEC MDR1 KnockoutCells [MDR1 knock out (-/-)]

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NACRES:
NA.81

biological source

human female renal (proximal convoluted tubule epithelial cells)

Quality Level

form

liquid

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... ABCB1(5243)

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MTOX1080MTOX1019MTOX1004
OMIM accession no.

171050

OMIM accession no.

602608

OMIM accession no.

-

OMIM accession no.

171050, 603756

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Gene Information

human ... ABCB1(5243)

Gene Information

human ... SLC22A2(23512)

Gene Information

human ... ABCB1(5243)

Gene Information

human ... ABCB1(5243), ABCG2(9429)

form

liquid

form

liquid

form

liquid

form

liquid

General description

Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and α-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, γ-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.

Application

Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture.

RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors.

RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate® in both toxicity and barrier integrity assays (( Z742750).

RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01).

Features and Benefits

Sigma′s human RPTEC control SA7K clone cells and knockout (KO) cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making them ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC KO cells
  • Sigma′s human RPTEC MDR1 (P-gp) Knockout (KO) allows investigations of drug transporter interactions involving P-gp in the kidney.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Other Notes

ADME/Tox cell lines license. These products are covered by the “Consumable Vial” License as described in Exhibit 1.

Legal Information

These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.
OrganoPlate is a registered trademark of MIMETAS B.V.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

AIM Biotech’s easy-to-use modular platform for conducting 3D cell culture into your research. The AIM Biotech organ-on-a-chip enables you to run simple assays or create more complex microphysiological systems. The versatile platform is suitable for a wide variety of cell types and is customizable for your specific needs.

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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