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MTOX1304

Sigma-Aldrich

Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells

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Synonym(s):
MDCKII MDR1 KO hBCRP knockin
NACRES:
NA.81

biological source

canine kidney (cocker spaniel)

Quality Level

form

liquid

storage temp.

−196°C

Gene Information

human ... ABCG2(9429)

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vibrant-m

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Canine MDCKII Wild Type Cells

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MTOX1001

MDR1 Knockout Caco-2 Cells

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Gene Information

human ... ABCG2(9429)

Gene Information

human ... ABCG2(9429)

Gene Information

-

Gene Information

human ... ABCB1(5243)

form

liquid

form

liquid

form

liquid

form

liquid

Application

See technical bulletin for detailed protocols

Features and Benefits

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard BCRP substrates.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

Exhibit 1: ADME/Tox cell lines license

MDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
Dominik Gartzke et al.
Pharmaceutical research, 32(6), 2060-2071 (2014-12-20)
To investigate whether it is possible to specifically suppress the expression and function of endogenous canine P-glycoprotein (cPgp) in Madin-Darby canine kidney type II cells (MDCKII) transfected with hPGP and breast cancer resistance protein (hBCRP) by zinc finger nuclease (ZFN)

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