MilliporeSigma
All Photos(1)

Documents

N2783

Sigma-Aldrich

Nitric Oxide Synthase, Inducible from mouse

recombinant, expressed in E. coli, buffered aqueous solution

Sign Into View Organizational & Contract Pricing

Synonym(s):
Inducible Nitric Oxide Synthase, NOS II, iNOS, macNOS
Enzyme Commission number:
MDL number:
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

form

buffered aqueous solution

specific activity

≥4.0 units/mg protein

mol wt

130 kDa (homodimer)
130 kDa (subunit, homodimer)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Nos2(18126)

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200766N7782N9657
Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

UniProt accession no.

P29477

UniProt accession no.

P35228

UniProt accession no.

P29477

UniProt accession no.

Q06518

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Gene Information

mouse ... Nos2(18126)

Gene Information

mouse ... Nos2(18126)

Gene Information

mouse ... Nos2(18126)
rat ... Nos2(24599)

Gene Information

human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)

General description

Inducible nitric oxide synthase (iNOS), also known as inflammatory nitric oxide synthase, is a calcium independent isoenzyme, involved in synthesis of nitric oxide (NO). It is a soluble enzyme encoded by the gene mapped to mouse chromosome 11. iNOS is active in dimeric form and its activity is induced by cytokines and various other stimuli. iNOS is expressed in various inflammatory conditions.

Application

Nitric Oxide Synthase, Inducible from mouse has been used in immunohistochemical studies. It is also used to evaluate the therapeutic efficacy of inducible nitric oxide synthase (NOS) on reperfusion-induced microcirculatory alterations and hemodynamic adverse effects in the microvasculature of skeletal muscle.

Biochem/physiol Actions

Tumor-derived inducible nitric oxide synthase (iNOS) plays a vital role in stimulating tumor growth and vessel maturation. Therefore, it is considered to be a potential therapeutic target for anti-vascular cancer therapies. Unchecked activity of iNOS leads to overproduction of nitric oxide (NO), which is toxic for living cells. iNOS activity can be controlled at both transcription and translational level by regulating protein stability, dimerization, phosphorylation, cofactor binding and availability of oxygen and L-arginine as substrates. iNOS plays a vital role in excisional wound repair and exhibits gene therapy strategy to advance wound healing process in iNOS-deficient conditions such as diabetes and steroid treatment.
NOS is responsible for the biosynthesis of nitric oxide from L-arginine. iNOS is not calcium/calmodulin dependent and has a Km = 16 μM for L-arginine.

Unit Definition

One unit will produce 1.0 μmol of nitric oxide per minute at 37 °C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 0.15 mM NADPH, 4.5 μM oxyhemoglobin, 18 μM tetrahydrobiopterin and 180 μM DTT.

Physical form

Solution in 50 mM HEPES, pH 7.4, with 10% glycerol, 8 μM tetrahydrobiopterin

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Mapping of the gene for inducible nitric oxide (NO) synthase of mouse macrophages to chromosome 11, close to Evi-2, nu, and Idd-4.
Jenkins NA
Genomics, 19(2), 402-404 (1994)
Investigating the role of tumour cell derived iNOS on tumour growth and vasculature in vivo using a tetracycline regulated expression system.
Papaevangelou E
International Journal of Cancer. Journal International Du Cancer, 138(11), 2678-2687 (2016)
P A Marsden et al.
FEBS letters, 307(3), 287-293 (1992-08-03)
The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase. However, recent cloning and molecular characterization of NO synthase from bovine endothelial cells indicated the existence of a family
Enhancing Nitric Oxide Bioavailability via Exogen Nitric Oxide Synthase and L-Arginine Attenuates Ischemia-Reperfusion-Induced Microcirculatory Alterations.
Engel H
Annals of Plastic Surgery (2014)
H Luss et al.
Journal of molecular and cellular cardiology, 29(4), 1153-1165 (1997-04-01)
There is evidence that nitric oxide (NO) may mediate some of the functional myocardial changes caused by bacterial LPS and inflammatory cytokines. The expression of the inflammatory or inducible NO synthase (iNOS) in human cardiac myocytes, however, has not been

Articles

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service